head of
- Puerto Rico Global Learning Observations to Benefit Environmetn Director 1996 -
- Science on Wheels Director 1991 - 2013
- Contact Info
Lopez-Garriga, Juan
Positions
- Professor, Department of Chemistry , College of Arts and Sciences
- Affiliation
- Publications
- Research
- Service
- Background
- Contact
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Affiliation
member of
- Puerto Rico Academy of Arts and Sciences Member 2005 -
- ACS Puerto Rico Chapter Councilor 2012 - 2013
Publications
selected publications
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academic article
- A highly efficient screening methodology for protein crystallization based on the counter-diffusion technique. J. Appl. Crystallogr. . 2013
- Hydrogen Sulfide Activation in Hemeproteins: The Sulfheme Scenario. J. Inorg. Biochem.. 2013
- Reactivity and dynamics of H2S, NO and O2 interacting with hemoglobins from Lucina pectinata. Biochemistry. 2013
- Electrochemistry of Hemoglobin I from Lucina pectinata immobilized on a modified gold electrode with 3-mercaptopropionic acid. Int. J. Mat. Biomat. App. . 2:29-36. 2012
- Tyrosine B10 triggers a heme propionate hydrogen bonding network loop with Glutamine E7 moiety. Biochemical and Biophysical Research Communications. 424:771-776. 2012
- Using a Reduced Dimensionality Model to Compute the Thermodynamic Properties of Finite Polypeptide Aggregates. Journal of Biological Physics. 38:383-395. 2012
- Hydrogen Sulfide and hemeproteins: knowledge and mysteries. Antioxidants & Redox Signaling Forum Review. 15:393-404. 2011
- Extreme Differences between Hemoglobins I and II of the Clam Lucina pectinalis in Their Reactions with Nitrite. BBA-Proteins and Proteomics. 1804:1988-1995. 2010
- Recombinant hemoglobin II from Lucina pectinata: A large-scale method for hemeprotein expression in E. coli. The Protein Journal. 29:143-151. 2010
- Structural determinants for the formation of sulfhemeprotein complexes. Biochemical and Biophysical Research Communications. 400:489-492. 2010
- Two-Step Counterdiffusion protocol for the crystallization of Hemoglobin II from Lucina pectinata in the pH range 4 to 9. Acta Cryst. F. F66:264-268. 2010
- Crystallization and Diffraction Patterns of the Oxy and Cyano Forms from the Lucina pectinata Hemoglobins Complex. Acta Cryst. F. 65:25-28. 2009
- Factors Controlling the Reactivity of Hydrogen Sulfide with Hemeproteins. Biochemistry. 48:4881-4894. 2009
- Characterization of the full length mRNA coding L. pectinata HBIII revealed an alternative polyadenylation site. Gene. 410:122-128. 2008
- Effects of Active Site Mutations in Hemoglobin I from Lucina Pectinata: a Molecular Dynamic Study. Molecular Simulation. 34:715-725. 2008
- Immobilization of HbI from Lucina Pectinata in Hydrophilic Polymer Networks for the Development of a Hydrogen Sulfide Biosensor. Journal of Applied Polymer Science. 107:881-890. 2008
- Immobilization of myoglobin from horse skeletal muscle in hydrophilic polymer networks. Journal of Applied Polymer Science. 107:881-890. 2008
- Structure and ligand selection of hemoglobin II from L. pectinata. J. Biol. Chemistry. 283:9414-9423. 2008
- Heme pocket geometry of L. pectinata hemoglobin II restricts nitric oxide and peroxide entry: A model of ligand control for the design of a stable oxygen carrier. Biochemistry. 46:10451-10460. 2007
- Lucina pectinata full-length cDNA sequence of hemoglobin III. Genbank. 2007
- Structure and ligand selection of hemoglobin II from Lucina pectinata. RCSB PDB. 2007
- Capillary Cristallization and molecular replacement solution of Hemoglobin II of the Clam Lucina Pectinata. Acta Cryst. F. 62:196-199. 2006
- Hemoglobin I from Lucina pectinata: A Model for distal heme-ligand control. Biochim. Biophys. Acta. 1764:758-765. 2006
- Hydrogen bonding conformations of Tyrosine B10 tailor the hemeprotein reactivity of ferryl species. Journal of Biological Inorganic Chemistry (JBIC). 11:334-342. 2006
- Sulfide-binding hemoglobins: Effects of mutations on the dynamics. Biophys. J. . 91:1698-1709. 2006
- Tyrosine B10 and Heme-ligand Interactions of Lucina pectinata Hemeglobin II: Control of Heme Reactivity. Biochim. Biophys. Acta. 1747:195-203. 2005
- Functional Characterization of the Purified Holo form of Hemoglobin I from Lucina pectinata Over-Expressed in Escherichia coli. The Protein Journal. 23:239-245. 2004
- High-level production of recombinant wild type and mutant sulfide-reactive hemoglobin I from L. pectinata in E. coli. Protein Expression Purif.. 38:184-195. 2004
- Time-resolved visible and infrared study of the cyano complexes of Myoglobin and Hemoglobin I from Lucina pectinata. Biophys. J. . 87:1323-11. 2004
- Ultrafast Studies of Ferric Complexes of Hemoglobin I from Lucina pectinata. Femtochemistry and Femtobiology. 395-398. 2004
- Lucina pectinata, full-length cDNA sequence of hemoglobin II . Genbank. 2003
- The cDNA-Derived Amino Acid Sequence of Hemoglobin II from Lucina Pectinata. J. Protein. Chem.. 22:683-690. 2003
- Evidence for a Nonhydrogen Bonded Compound II in the Cyclic Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroide. Biopolymers. 62:176-185. 2002
- Investigation of the Carbonmoxide and Oxy Hemoglobin I Complexes from Lucina pectinata in Femtosecond Transient Absorption. Femtochemistry and Femtobiology. 720-730. 2002
- Expression and Purification of Recombinant Hemoglobin I from Lucina pectinata. J. Protein. Chem.. 20:311-331. 2001
- Formation Compounds I and Compound II Ferryl Species in the Reactions of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide. Arch. Biochem. Biophys.. 390:304-308. 2001
- Formation of Compound I and Compound II Ferry] Species in the Reaction of Hemoglobin I from Lucina pectinata with Hydrogen Peroxide. Arch. Biochem. Biophys.. 390:304-308. 2001
- Hands-on Chemistry Demonstrations and the Global Learning and Observations to Benefit the Environment (GLOBE) Program. Journal of Chemical Education. 77:1586-1589. 2000
- Natrinema thermotolerans 16S rRNA sequence. Genbank. 2000
- Natrinema thermotolerans sp. nov., a halophilic archaeon from Puerto Rico. Inter. J. Syst. and Evol. Microbiol.. 50:1065-1071. 2000
- Lucina pectinata full-length cDNA sequence of hemoglobin. Genbank. 1999
- Resonance Raman Studies of the Heme-Ligand Active Site of HbI from Lucina pectinata. Biospectroscopy. 5:289-301. 1999
- The cDNA-Derived Amino Acid sequence of HbI from Lucina pectinata. J. Protein. Chem.. 18:831-836. 1999
- Halogeometricum borinquense gen. nov., sp. Nov., a Novel Halophilic Archaen from Puerto Rico. Inter. J. Syst. Bacteriol. . 48:1305-1312. 1998
- Halogeometricum borinquense, 16S rRNA sequence . Genbank. 1998
- Orientation of the Heme Vinyl Groups in the H2S-Binding HbI from Lucina pectinata. Biospectroscopy. 4:311-326. 1998
- Resonance Raman of Heme-Ligand Complexes of HbI from L. pectinata. Proceedings XVI Raman Spectroscopy. 294-295. 1998
- Unusual Flexibility of the Heme Propionates in the Hydrogen Sulfide-Binding HbI of L. pectinata. J. Am Chem. Soc.. 120:9312-9317. 1998
- New Record of Extremely Halophilic Archaebacteria for Puerto Rico and the Caribbean. Caribbean Journal of Science . 33:98-104. 1997
- Science on Wheels: A Coherent Link Between Educational Cultures. Journal of Chemical Education. 74:1346-1349. 1997
- Structural characterization and dynamics events in hemoglobin I from Lucina pectinata: Unusual conformation of propionates and vinyl's heme peripheral groups. Spectrosc. Biol. Mol.: Trends. 79-80. 1997
- Control of CO Binding States and Dynamics in Hbl of Lucina pectinata by Aromatic Residues. Inorg. Chim. Acta.. 243:161-166. 1996
- Resonance Raman Studies of AquoHbI form Lucina pectinata. Proceedings XIV Raman Spectroscopy. 438-439. 1996
- Time-Resolved Vibrational Spectroscopy. Inter-American Photochemical Society. 19:20-23. 1996
- Characterization of Ligand Binding and Electron Transfer Properties Hbl from L. pectinata. J. Inorg. Biochem.. 59:447. 1995
- Structure and Dynamic Behavior of Heme-CO of Hemoglobin I from Lucina pectinata. Spectroscopy of Biological Molecules. 233-234. 1995
- Spectroscopic Evidence for the Rehybridization mechanism Upon Protonation of Retinal Schiff's Bases. Proceedings XIV Raman Spectroscopy. 98-99. 1994
- Photodissociation and recombination of carbonmonoxy cytochrome oxidase: Dynamics from picoseconds to kiloseconds. Biochemistry. 32:12013-12024. 1993
- Resonance Raman Characterization of Free-Base Porphycene and Ni(II), Co(II), Fe(II) and Fe(III) Porphycene Complexes. J. Am Chem. Soc.. 113:127-134. 1991
- Metal-Ligand Vibrations of Cyanoferric Myeloperoxidase and Cyanoferric Horseradish Peroxidase: Evidence for a Constrained Heme Pocket for Myeloperoxidase. Biochemistry. 29:9387-9395. 1990
- Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Bovine Cytochrome Oxidase at 300K. J. Am Chem. Soc.. 111:7657-7659. 1989
- Metal-Ligand Vibrations of Cyanoferric Myeloperoxidase and Cyanoferric Horseradish Peroxidase: Evidence for a Constrained Heme Pocket for Myeloperoxidase. Proceedings XI on Raman Spectroscopy. 980-982. 1988
- Factors influencing the C=N Stretching Frequency in Neutral and Protonated Schiff's Bases. J. Am Chem. Soc.. 108:7241-7251. 1986
- Increase in the C=N Stretching Frequency Upon Complexation of Trans-Retinylidene n-butylamine and other Schiff's Bases with General Lewis Acid. Proceedings X on Raman Xpectroscopy. 1434-1435. 1986
- Increase in the C=N Stretching Frequency Upon Complexation of Trans-Retinylidene n-butylamine with General Lewis Acid. J. Am Chem. Soc.. 108:7131-7133. 1986
- Rehybridization of the C=N Bond Upon Protonation of Methylimine Increases the C=N Stretching Force Constant. J. Am Chem. Soc.. 108:7251-7254. 1986
- Factors Influencing the C=N Stretching Force Constant in Neutral and Protonated Schiff's Bases. Biophys. J. . 96. 1985
- The Raman Spectra of Selenomethionine and Selenocystine. J. Inorg. Biochem.. 14:177-188. 1981
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article
- Haloterrigena thermotolerans sp. nov., a Halophilic Archaeon from Puerto Rico. Int. J. Syst. Evol. Micro.. 1065-1071. 2000
- Halogeometricum borinquense gen. nov., sp. nov., a Novel Halophilic Archaeon from Puerto Rico. Int. J. Syst. Bact.. 1305-1312. 1998
- New Isolates of Extremely Halophilic Archaebacteria (Halobacteria) from Puerto Rico and the Caribbean. Caribbean Journal of Science. 98-104. 1997
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conference paper
- Center for Resources in General Education (CIVIS): Towards Student Success in General and STEM Education. ASEE/IEEE Frontiers in Education. 2009
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conference poster
- Hydrogen Sulfide Limits Amyloids Development in Myoglobin and Hemoglobin. 53rd ACS Junior Technical Meeting (JTM) and 38th Puerto Rico Interdisciplinary Scientific Meeting (PRISM). 2019
presentations
-
invited talk
- Cytochrome a3 is Low Spin Following Photodissociation of CO from Reduced Cytochrome Oxidase, Speaker
- Dynamics Photoligands in Cytochrome Oxidase, Speaker, Symposium Apply Chemistry, UPR Arecibo
- Expository Program K20+: Enhancing Chemistry and Technology Education, Speaker, Holly Cross University, Pennsylvania
- Expository Programs (II): Enhancing Science Education to the Public, Speaker, Parque Ciencias de Granada, Spain
- Expository Programs: An Approach to Enhance Science Education, Speaker, Caribbean Institute for Bio-Exploration (CIBEX)
- Expository Programs: An Approach to Enhance Science Education. Expanding opportunities for K-12, Speaker, Rutgers University
- Expository Programs: Enhancing Chemistry and Technology Education, Speaker, Rutgers University
- Expository Programs: Enhancing Science Education to the Public, Speaker, Parque Ciencias de Granada, Spain
- GLOBE and Outreach programs in Puerto Rico, Speaker, Texas Science Summit, San Antonio TX
- GLOBE program in Puerto Rico: Implementation Panel, Speaker, VI Annual GLOBE Conference
- GLOBE: An International Educational Program , Speaker, Tercer Encuentro Regional de Educacion y Pensamiento, UPR Mayaguez
- GLOBE: Global Learning and Observations to Benefit the Environment, Speaker, 8va Conferencia Estatal para el Mejoramiento Profesional de los Maestros de Ciencia y Matematicas
- Graduates and Undergraduate Students Leadership in Pre-College Education, Speaker, University of Virgen Island in Saint Croix
- Halogenation Processes in Tropical Marine Biota, Speaker, Marine Science Department, UPR Mayaguez
- Heme - proteins structure and function, Speaker, Parque Ciencias de Granada, Spain
- Hemoglobin I from Lucina pectinata: Structure, Function and Technological Applications, Speaker, Chemistry Department, Virginia Commonwealth University
- Hemoglobins of L. pectinata: Structure and function relation, Speaker, CSIC, Granada Spain
- Hydrogen Sulfide Activation in Hemeproteins, Speaker, The Sulfheme Scenario
- Hydrogen Sulfide: Transport and Reactivity in Hemeproteins, Speaker, Department of Physiology and Biophysics Albert Einstein College of Medicine Bronx
- Importance of Re-Training K-12 Teachers, Speaker, Carmen Salas, Jayuya
- Interaction of Heme Proteins with Compounds from Tropical Marine Environments: A Bioactive Probe, Speaker, Marine Natural Products, La Parguera PR
- International Activities in Research and Education, Speaker, GK-12 Conference - NSF
- Lasers and Chemistry, Speaker
- Ligand in Cytochrome Oxidase, Speaker, Catholic University of Puerto Rico
- Novel Applications for FT Raman, Speaker, Second Pan American Chemical Congress
- Orientation of the Fe-CO Moiety in Heme-CO Complexes, Speaker, XX Latin American Chemical Congress
- Rehybridization Mechanism Nitrogen Electron Lone Pair, Speaker, University of Puerto Rico Rio Piedras
- Rehybridization of the C=N Bond Upon Protonation of Schiff's Bases Increases the Stretching Frequency, Speaker, Los Alamos National Laboratory
- Science Shows and Chemistry, Speaker
- Science on Wheels, Speaker, National Chemistry, UPR Ponce
- Science on Wheels Educational Center: An integration of Outreach Programs from Industry, Government, Academia, and Scientific Societies, Speaker, 7th Annual GLOBE Conference
- Spectroscopic Techniques Applied to Biotechnology, Speaker, 13th International Conference in Chemical Education, San Juan, PR
- Structural Events in Hbl from Lucina pectinata, Speaker, Barcelona Scientific Park, Spain
- Structural Events in Hemoglobin I Ligand Complexes of Lucina pectinata, Speaker, XII International Conference on Invertebrates Dioxygen Binding Proteins
- Structural Events of Hemoglobins Ligand Complexes from Lucina Pectinata, Speaker, Chemistry Division, Argonne National Laboratory
- Structural Relations in Cytochrome Oxidase, Speaker, Ponce Medical School, Ponce PR
- Structure and Technological Applications of Hbl from L. pectinata, Speaker, Department of Physiology and Biophysics Albert Einstein College of Medicine Bronx
- Students in Outreach programs, Speaker, Duke Marine/Freshwater Biomedical Center
- The GLOBE program in Puerto Rico, Speaker, Centro de Investigacion y Documentacion Educativa, Ministerio de Educacion y Ciencia, Madrid
- The NSF GK-12 of the University of Puerto Rico at Mayaguez, Speaker, Centro de documentacion experimentacion en ciencias y tecnologia, Barcelona, Spain
- Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Bovine Cytochrome Oxidase at 300K, Speaker, Gordon Research Conference "Metals in Biology"
- Time-Resolved Infrared Observation of a CuB+-CO Phototransient in Cytochrome Oxidase, Speaker
- Time-Resolved Infrared Spectroscopy and Proteinquakes, Speaker, Laser EPSCoR
- Time-resolved IR Spectroscopy, Speaker, NSF-International Workshop
- Transient Absorbance, Time-Resolved Infrared and Resonance Raman Studies: Photodissociation of ligands from Cytochrome Oxidase, Speaker, Harvard Medical School, Wellman Laboratory
- Undergraduate Research in Chemistry, Speaker
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presentation
Research
principal investigator on
other research activities
- Acquisition of a Terahertz Imaging and Spectroscopic to Enhance Research and Education Activities at UPRM [Not Funded] Co-PI
- Center for Research in Protein Structure, Function and Dynamics PI
- Polymer Confinement of Protein Crystals:an InnovativeApproach to Controlled Drug Release Collaborator
- RISE-2-BEST program at UPRM: Enhancing Biomedical Sciences and Biomedical Engineering in Science and Technology PI
- Selective Reactivity of H2S with GlnE7 and HisE7 in Hemoglobins: Implications Organimss PI 2009 - 2014
- Center for Education and Training in Agricultural and Related Sciences (CETARS), Division of Community and Education Co-PI 2010 - 2013
- Advancing Competitive Biomedical Research in Puerto Rico PI 2009 - 2013
- Wisconsin - Puerto Rico Partnership for Research and Education in Materials Co-PI 2009 - 2013
- Center for Resources in General Education: Towards Student Success in General and STEM Education at UPRM Co-PI 2008 - 2013
Service
organizer of event
- 37th Senior Meeting, ACS-Local Session - 2013
- 36th Senior Meeting, ACS-Local Session - 2012
- RISE in biomedical and bioengineering - 2012
- First Arts, Sciences and Engineering Festival and Science on Wheels Educational Center 20th Anniversary - 2011
- Symposium Structural Dynamics of Chemical and Biological Systems, Session II of II - 2011
- First International crystallization competition high school students Puerto Rico-Spain - 2009
- GK-12 Conference - NSF - 2009
- 30th Senior Meeting, ACS-Local Session - 2006
- 41st Junior Technical Meeting, ACS-Local Session - 2006
- Science on Wheels Educational Center 15th Anniversary - 2006
- 29th Senior Meeting, ACS-Local Session - 2005
- Second Colloquium in Protein Structure, Function and Dynamics - 2005
- First Colloquium in Protein Structure, Function and Dynamics: Frontiers in Protein Spectroscopy - 2004
- Summer seminar series in Protein Structure, Function and Dynamics - 2003
professional service activities
- University of Wisconsin, Milwaukee Honorary Associate Professor 2010 -
- Academia de Investigacion Para la Facultad y Postdoctorales Mentor
Background
education and training
- Ph.D. in Physical Chemistry, Michigan State University , Factors Influencing the C=N Stretching Frequency in Neutral and Protonated Schiff's Bases 1981 - 1986
- M.Sc. in Chemistry 1980
- B.Sc. in Chemical Engineering 1975
- Los Alamos National Laboratory 1988 - 1989
- Michigan State University 1986 - 1988
awards and honors
- Adopted Son of the City of Mayaguez, conferred by Mayaguez, Puerto Rico, 2013
Contact
full name
- Juan Lopez Garriga